Coding

Part:BBa_K1732019:Design

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-16)


E0040-HIS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


Design Notes

6X His Tag was added for easier protein purification.


Source

The GFP sequence was the Green Fluorescent protein derived from jellyfish Aequeora victoria wild-type. It was the same sequence used in the iGEM 2015 interlab study.

References